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Monoamines in the pedal plexus of the land snail Megalobulimus oblongus (Gastropoda, Pulmonata) BJMBR
Faccioni-Heuser,M.C.; Zancan,D.M.; Achaval,M..
In molluscs, the number of peripheral neurons far exceeds those found in the central nervous system. Although previous studies on the morphology of the peripheral nervous system exist, details of its organization remain unknown. Moreover, the foot of the terrestrial species has been studied less than that of the aquatic species. As this knowledge is essential for our experimental model, the pulmonate gastropod Megalobulimus oblongus, the aim of the present study was to investigate monoamines in the pedal plexus of this snail using two procedures: glyoxylic acid histofluorescence to identify monoaminergic structures, and the unlabeled antibody peroxidase anti-peroxidase method using antiserum to detect the serotonergic component of the plexus. Adult land...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pedal plexus; Catecholamines; Serotonin; Glyoxylic acid; Immunohistochemistry; Gastropod.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700014
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The terrestrial Gastropoda Megalobulimus abbreviatus as a useful model for nociceptive experiments: effects of morphine and naloxone on thermal avoidance behavior BJMBR
Achaval,M.; Penha,M.A.P.; Swarowsky,A.; Rigon,P.; Xavier,L.L.; Viola,G.G.; Zancan,D.M..
We describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Snail; Thermal avoidance; Nociception; Morphine; Naloxone.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100012
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